Tutorial |
Description |
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Compute Cluster |
Xanadu Cluster (SLURM) |
Understanding the UConn Health Cluster (Xanadu) |
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Array Job Submission |
Instructions to submit array Job on Xanadu |
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SLURM Resource Guidelines |
Requesting resource allocation |
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Package Installation in Home Directory |
Python and R packages |
Install Python and R packages in User’s Home Directory |
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File Formats |
Common File Formats in Bioinformatics |
Bioinformatics File Formats Explained |
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Data Transfer and Management |
Data Download from Basespace (Illumina) |
Basespace data download tutorial |
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Data Transfer Outline |
File Transfer Instructions |
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Data Management Guidelines |
Data Management on Xanadu |
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RNA-Seq Guides |
Reference genome-based RNA-Seq |
Differential expression analysis a reference genome using HISAT2/HTSeq/DESeq2 |
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De novo transcriptome-based RNA-Seq |
Differential expression analysis using a de novo assembled and annotated transcriptome using Trinity/entap/kallisto/DESeq2 |
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Single-Cell RNA-Seq analysis |
Single-Cell RNA-Seq analysis (10X genomics, CellRanger) |
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Prokaryote RNA-Seq |
EDGE-pro tutorial (with Listeria reference genome) |
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Model Plant RNA-Seq |
Differential expression analysis with Arabidopsis using HISAT2/StringTie/Ballgown. |
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Structural Annotation |
Structural Annotation |
Structural Annotation for Assembled Genomes (BRAKER/MAKER/EnTAP/gFACS/GffCompare) |
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Genome Assembly |
Genome Size Estimation |
Estimate genome size with k-mer based analysis of short read data (Jellyfish/R/GenomeScope) |
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Genome Assembly and Assessment |
Genome assembly and evaluation using short reads, long reads, and a hybrid approach |
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Variant Detection |
Variant Detection |
Detecting variation using short read data mapped to a reference genome (GATK, Freebayes, bcftools) |
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RADseq Analysis |
Detecting variation in restriction-site-associated DNA using de novo assembly and reference-based methods (Stacks, Freebayes) |
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Analysis Packages |
Geneious |
Geneious local install and login information |
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PacBio |
PacBio SMRT Portal |
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